You didn't specify which version of LLBLGen are you using, which tenmplateSet and if you are using the Low Level APIs, Linq or QuerySPec.
If you are using the low level APIs, then using a DBFunctionCall
Example:
new DbFunctionCall("SUBTIME({0}, '1:30')", new object[] {YourEntityFields.TimeField });
This is the Expression that you can use in a filter or in a select as shown in the above link.
For QuerySpec and Linq there is an extra step to define a function mapping.
But if you can convert the time to minutes or hours, then you can use the built-in mapped function AddMinutes() or AddHours() and provide a negative value.